Characterization of human sperm

Characterization of Mammalian ADAM2 and Its Absence from Human Sperm

The members of the ADAM (a disintegrin and metalloprotease) family are membrane-anchored multi-domain proteins that play prominent roles in male reproduction. ADAM2, which was one of the first identified ADAMs, is the best studied ADAM in reproduction. In the male germ cells of mice, ADAM2 and other ADAMs form complexes that contribute to sperm-sperm adhesion, sperm-egg interactions, and the mi...

Characterization of the kisspeptin system in human sperm

Proteolytic release and partial characterization of human sperm-surface glycopeptides.

Sperm-surface glycopeptides were obtained from intact sperm membranes after proteolytic release by different enzymatic treatments such as autoproteolysis, trypsin, papain and pronase. Glycopeptides were isolated, their properties and composition were examined, and their monosaccharide and amino acid constituents were characterized. The monosaccharides identified were fucose, mannose, galactose,...

Cryobanking of human sperm.

The primary use of frozen semen has been for the treatment of male infertility, in which case a 'donor's' semen may be used for insemination independently of time and place. An increase in demand for semen by infertile couples, who represent about 30 per cent of all marriages in the United States, was a contributing factor to the rapid growth of commercial semen banking. Another factor was the ...

Effects of combined magnetic fields on human sperm parameters

Background: In previous investigations, it has been clarified that electromagnetic fields (ELF) can cause some changes in cellular behavior. The aim of this prospective study was to investigate the effect of magnetic field (MF) on human sperm parameters of motility, morphology, and viability. Materials and Methods: Semen samples were collected from 12 fertile men, and were allowed to l...